NOS3 ELISA Kit (Mouse) (OKCD00667)

Catalog No: OKCD00667 (Formerly GWB-KBBUL1)

Size:96WELLS

Mouse Nos3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	85-101%	90-97%	90-104%	87-103%
  EDTA plasma(n=5)	92-105%	78-105%	90-98%	86-94%
  heparin plasma(n=5)	95-104%	94-105%	96-103%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelial NOS (eNOS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelial NOS (eNOS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelial NOS (eNOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Endothelial NOS (eNOS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-91	88
  EDTA plasma(n=5)	87-104	93
  heparin plasma(n=5)	95-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelial NOS (eNOS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelial NOS (eNOS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Nos3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Nos3 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated Nos3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.57 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 3, Endothelial (NOS3). No significant cross-reactivity or interference between Nitric Oxide Synthase 3, Endothelial (NOS3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Nos3
• Gene Full Name: nitric oxide synthase 3, endothelial cell
• Alias Symbols: 2310065A03Rik;cNOS;constitutive NOS;e;ec;ecNOS;EC-NOS;endothelial nitric oxide synthase;endothelial NOS;eNOS;nitric oxide synthase, endothelial;No;NOS type III;Nos-3;NOSIII.
• NCBI Gene Id: 18127
• Protein Name: Nitric oxide synthase, endothelial
• Description of Target: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.
• Uniprot ID: P70313
• Protein Accession #: NP_032739
• Nucleotide Accession #: NM_008713.4
• Protein Size (# AA): 1202