NOS2 ELISA Kit (Rat) : 96 Wells (OKEH00555)

Catalog No: OKEH00555

Size:96T

Rat NOS2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NOS2 ELISA Kit (Rat) (OKEH00555) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat NOS2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NOS2 and incubated. The NOS2 found in the sample or standards competes with the biotinylated NOS2 for limited binding sites on the immobilized anti-Rat NOS2 antibody. Excess unbound biotinylated NOS2 and sample or standard NOS2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NOS2 captured in the well and inversely proportional to the amount of NOS2 which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.1% (n = 20)
  Mean Inter-assay CV%: < 10.5% (n = 20)
• ELISA Kit Component:
  • Anti-NOS2 Microplate: 96 Wells (12 x 8 Well strips)
  • NOS2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X NOS2-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.78nmol/L
• Specificity: Natural and recombinant Rat Nitric oxide synthase, inducible
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Nos2
• Gene Full Name: nitric oxide synthase 2
• Alias Symbols: inducible nitric oxide synthase;inducible NO synthase;inducible NOS;iNos;nitric oxide synthase 2, inducible, macrophage;nitric oxide synthase, inducible;nitric oxide synthase, inducible-like;NOS type II;Nos2a;peptidyl-cysteine S-nitrosylase NOS2.
• NCBI Gene Id: 24599
• Protein Name: Q06518
• Description of Target: Nitric oxide synthase, inducible
• Uniprot ID: https://www.uniprot.org/uniprot/Q06518
• Protein Accession #: NP_036743.3
• Nucleotide Accession #: NM_012611.3
• Protein Size (# AA): 1147
• Molecular Weight: 131 kDa