NMES1 ELISA Kit (Mouse) (OKEH04237)

Catalog No: OKEH04237

Size:96T

Mouse NMES1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Nmes1 ELISA Kit (Mouse) (OKEH04237) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nmes1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nmes1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nmes1 captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.9% (n = 20)
  Mean Inter-assay CV%: <= 9.5% (n = 20)
• ELISA Kit Component:
  • Nmes1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Nmes1 Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Nmes1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.172 ng/mL
• Specificity: Natural and recombinant Mouse Normal mucosa of esophagus-specific gene 1 protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AA467197
• Gene Full Name: expressed sequence AA467197
• Alias Symbols: mir-147;NME;Nmes1;normal mucosa of esophagus specific 1;normal mucosa of esophagus-specific gene 1 protein.
• NCBI Gene Id: 433470
• Protein Name: Q810Q5
• Description of Target: Normal mucosa of esophagus-specific gene 1 protein
• Uniprot ID: https://www.uniprot.org/uniprot/Q810Q5
• Protein Accession #: NP_001004174.1
• Nucleotide Accession #: NM_001004174.1
• Protein Size (# AA): 83
• Molecular Weight: 10 kDa