NLRC4 ELISA Kit (Human) : 96 Wells (OKEH01101)

Catalog No: OKEH01101

Size:96T

Human NLRC4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NLRC4 ELISA Kit (Human) (OKEH01101) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NLRC4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NLRC4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NLRC4 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 8.2% (n = 20)
• ELISA Kit Component:
  • NLRC4 Microplate: 96 Wells (12 x 8 Well strips)
  • NLRC4 Lyophilized Standard: 2
  • 100X Biotinylated NLRC4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 39 pg/mL
• Specificity: Natural and recombinant Human NLR family CARD domain-containing protein 4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NLRC4
• Gene Full Name: NLR family CARD domain containing 4
• Alias Symbols: AIFEC;CARD, LRR, and NACHT-containing protein;CARD12;caspase recruitment domain family, member 12;caspase recruitment domain-containing protein 12;CED-4-like protein Clan;CLAN;CLAN1;CLANA;CLANB;CLANC;CLAND;CLR2.1;FCAS4;Ice protease-activating factor;ICE-protease activating factor;IPAF;NLR family CARD domain-containing protein 4;NOD-like receptor C4;nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4.
• NCBI Gene Id: 58484
• Protein Name: Q9NPP4
• Description of Target: NLR family CARD domain-containing protein 4
• Uniprot ID: https://www.uniprot.org/uniprot/Q9NPP4
• Protein Accession #: NP_001186067.1
• Nucleotide Accession #: NM_001199138.1
• Protein Size (# AA): 1024
• Molecular Weight: 116 kDa