NLK Antibody (OAGA00616)

Catalog No: OAGA00616

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
NLK antibody
OAGA00616 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA00616 diluted at 1:1000

NLK antibody [N3C3] detects NLK protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NLK antibody [N3C3] (OAGA00616) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NLK(OAGA00616) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC, WB
• Additional Information: Background: Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
  Positive Controls: A431, H1299, HeLa, mouse brain
  Cellular Localization: Nucleus (By similarity), Cytoplasm (By similarity)
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 234 and 527 of NLK (Uniprot ID#Q9UBE8)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: NLK
• Gene Full Name: Serine/threonine-protein kinase NLK
• Alias Symbols: DKFZp761G1211 antibody, FLJ21033 antibody, NLK antibody, serine/threonine-protein kinase NLK antibody, nemo-like kinase antibody
• NCBI Gene Id: 51701
• Protein Name: Serine/threonine-protein kinase NLK
• Uniprot ID: Q9UBE8
• Protein Accession #: NP_057315.3
• Molecular Weight: 58 kDa