NID1 ELISA Kit (Human) (OKEH04109)

Catalog No: OKEH04109

Size:96T

Human NID1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NID1 ELISA Kit (Human) (OKEH04109) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NID1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NID1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NID1 captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.2% (n = 20)
  Mean Inter-assay CV%: <= 9.7% (n = 20)
• ELISA Kit Component:
  • Anti-NID1 Microplate: 96 Wells (12 x 8 Well Strips)
  • NID1 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • NID1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.067 ng/mL
• Specificity: Natural and recombinant Human Nidogen-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NID1
• Gene Full Name: nidogen 1
• Alias Symbols: enactin;entactin;epididymis secretory sperm binding protein;NID;NID-1;nidogen-1.
• NCBI Gene Id: 4811
• Protein Name: P14543
• Description of Target: Nidogen-1
• Uniprot ID: https://www.uniprot.org/uniprot/P14543
• Protein Accession #: NP_002499.2
• Nucleotide Accession #: NM_002508.2
• Protein Size (# AA): 1247
• Molecular Weight: 136 kDa