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Catalog No: OKCD02757
Size:96WELLS
Price: $625.00
SKU
OKCD02757
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Datasheets/ManualsPrintable datasheet for NGB ELISA Kit (Mouse) (OKCD02757)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)79-98%94-103%84-94%82-97%
EDTA Plasma (n=5)85-102%83-101%88-96%81-98%
Heparin Plasma (n=5)98-105%98-105%89-103%80-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroglobin (NGB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroglobin (NGB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroglobin (NGB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuroglobin (NGB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)86-10190
EDTA Plasma (n=5)92-10198
Heparin Plasma(n=5)95-103101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroglobin (NGB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroglobin (NGB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NGB Microplate96 Wells (12 x 8 Well strips)
NGB Lyophilized Standard2
100X Biotinylated NGB Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.142 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Neuroglobin (NGB). No significant cross-reactivity or interference between Neuroglobin (NGB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolNgb
Gene Full Nameneuroglobin
Alias Symbolsneuroglobin.
NCBI Gene Id64242
Protein NameNeuroglobin
Description of TargetInvolved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
Uniprot IDQ9ER97
Protein Accession #NP_071859.1
Nucleotide Accession #NM_022414.2
Protein Size (# AA)151
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