Catalog No: OKEH04030
Size:96T
Price: $425.00
SKU
OKEH04030
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NFKBIA ELISA Kit (Human) (OKEH04030) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NFKBIA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NFKBIA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NFKBIA captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.8% (n = 20)
Mean Inter-assay CV%: <= 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-NFKBIA Microplate96 Wells (12 x 8 Well Strips)
NFKBIA Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
NFKBIA Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human NF-kappa-B inhibitor alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNFKBIA
Gene Full NameNFKB inhibitor alpha
Alias SymbolsEDAID2;IkappaBalpha;I-kappa-B-alpha;IKBA;ikB-alpha;MAD-3;major histocompatibility complex enhancer-binding protein MAD3;NF-kappa-B inhibitor alpha;NFKBI;nuclear factor of kappa light chain gene enhancer in B-cells;nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha.
NCBI Gene Id4792
Protein NameP25963
Description of TargetNF-kappa-B inhibitor alpha
Uniprot IDhttps://www.uniprot.org/uniprot/P25963
Protein Accession #NP_065390.1
Nucleotide Accession #NM_020529.2
Protein Size (# AA)317
Molecular Weight36 kDa
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