NFKBIA Antibody (Phospho-Tyr305) (OAAB20439)

Catalog No: OAAB20439

Size:100ul

Western blot analysis of extracts from COS7 cells, treated with nocodazole (1ug/ml, 16hours), using IκB-α (Phospho-Tyr305) antibody (OAAB20439).

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using IκB-α (Phospho-Tyr305) antibody (OAAB20439).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Function: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Target Post-Translational Modification: Phospho-Tyr305
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:1000
  IHC: 1:50~100
• Reference: Haskill S.,et al.Cell 65:1281-1289(1991).
  Jungnickel B.,et al.J. Exp. Med. 191:395-402(2000).
  Liu B.,et al.Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
  Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  

Target Info

• Gene Symbol: NFKBIA
• Gene Full Name: NFKB inhibitor alpha
• Alias Symbols: IKBA, MAD-3, NFKBI, EDAID2
• NCBI Gene Id: 4792
• Description of Target: This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
• Uniprot ID: P25963
• Protein Accession #: NP_065390.1
• Nucleotide Accession #: NM_020529.2
• Molecular Weight: 36 kDa