NFKB1 ELISA Kit (Mouse) : 96 Wells (OKEH01301)

Catalog No: OKEH01301

Size:96T

Mouse NFKB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NFKB1 ELISA Kit (Mouse) (OKEH01301) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NFKB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NFKB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NFKB1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 3.8% (n = 20)
  Mean Inter-assay CV%: < 7.2% (n = 20)
• ELISA Kit Component:
  • NFKB1 Microplate: 96 Wells (12 x 8 Well strips)
  • NFKB1 Lyophilized Standard: 2
  • 100X Biotinylated NFKB1 Detector Antibody: 1 x 20 mL
  • 100X Avidin-HRP Conjugate: 1 x 60 uL
  • Sample Diluent: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.091 ng/mL
• Specificity: Natural and recombinant Mouse Nuclear factor NF-kappa-B p105 subunit
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Nfkb1
• Gene Full Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
• Alias Symbols: DNA-binding factor KBF1;EBP-1;NF kappaB1;NF-k;NF-kappaB;NF-kappaB p50;NF-kappaB1;NF-kappa-B1 p84/NF-kappa-B1 p98;NF-KB1;nuclear factor kappaB p50;nuclear factor NF-kappa-B p105 subunit;nuclear factor of kappa light chain gene enhancer in B-cells 1, p105;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1;p105;p5;p50;p50 subunit of NF kappaB;p50/p1;p50/p105.
• NCBI Gene Id: 18033
• Protein Name: P25799
• Description of Target: Nuclear factor NF-kappa-B p105 subunit
• Uniprot ID: https://www.uniprot.org/uniprot/P25799
• Protein Accession #: NP_032715.2
• Nucleotide Accession #: NM_008689.2
• Protein Size (# AA): 971
• Molecular Weight: 106 kDa