Catalog No: OKEH04591
Size:96T
Price: $525.00
SKU
OKEH04591
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology NFKB1 ELISA Kit (Human) (OKEH04591) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NFKB1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for NFKB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NFKB1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.23 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Nuclear factor NF-kappa-B p105 subunit | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | NFKB1 |
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Gene Full Name | nuclear factor kappa B subunit 1 |
Alias Symbols | CVID12;DNA-binding factor KBF1;EBP-1;KBF1;NFkappaB;NF-kappaB;NF-kappa-B1;NF-kappabeta;NF-kB;NF-kB1;NFKB-p105;NFKB-p50;nuclear factor kappa-B DNA binding subunit;nuclear factor NF-kappa-B p105 subunit;nuclear factor NF-kappa-B p50 subunit;nuclear factor of kappa light polypeptide gene enhancer in B-cells 1. |
NCBI Gene Id | 4790 |
Protein Name | P19838 |
Description of Target | Nuclear factor NF-kappa-B p105 subunit |
Uniprot ID | https://www.uniprot.org/uniprot/P19838 |
Protein Accession # | NP_001158884.1 |
Nucleotide Accession # | NM_001165412.1 |
Protein Size (# AA) | 968 |
Molecular Weight | 105 KDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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