Catalog No: OKCD00474 (Formerly GWB-KBBLK1)
Size:96WELLS
Price: $675.00
SKU
OKCD00474
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for NFIB ELISA Kit (Human) (OKCD00474)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor I/B (NFIB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor I/B (NFIB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor I/B (NFIB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nuclear Factor I/B (NFIB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor I/B (NFIB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor I/B (NFIB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NFIB Microplate96 Wells (12 x 8 Well Strips)
NFIB Lyophilized Standard2 x 10 ng
100X Biotinylated NFIB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nuclear Factor I/B (NFIB). No significant cross-reactivity or interference between Nuclear Factor I/B (NFIB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNFIB
Gene Full Namenuclear factor I B
Alias SymbolsCCAAT-box-binding transcription factor;CTF;HMGIC/NFIB;MACID;NF1-B;NF-I/B;NFI-B;NFIB2;NFIB3;NFI-RED;nuclear factor 1 B-type;nuclear factor 1/B;Nuclear factor I/B;TGGCA-binding protein.
NCBI Gene Id4781
Protein NameNuclear factor 1 B-type
Description of TargetRecognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Uniprot IDO00712
Protein Accession #NP_001177666
Nucleotide Accession #NM_001190737.1
Protein Size (# AA)420
Write Your Own Review
You're reviewing:NFIB ELISA Kit (Human) (OKCD00474)
Your Rating
We found other products you might like!