Catalog No: OKEH02447
Size:96T
Price: $625.00
SKU
OKEH02447
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NFE2L1 ELISA Kit (Human) (OKEH02447) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NFE2L1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NFE2L1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NFE2L1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 9.9% (n = 20)
ELISA Kit Component
ComponentAmount
NFE2L1 Microplate96 Wells (12 x 8 Well strips)
NFE2L1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated NFE2L1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity35 pg/mL
SpecificityNatural and recombinant Human Nuclear factor erythroid 2-related factor 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNFE2L1
Gene Full Namenuclear factor, erythroid 2 like 1
Alias Symbolsendoplasmic reticulum membrane sensor NFE2L1;LCR-F1;locus control region-factor 1;NFE2-related factor 1;NF-E2-related factor 1;NRF1;nuclear factor erythroid 2-related factor 1;nuclear factor, erythroid derived 2, like 1;protein NRF1, p120 form;TCF11;TCF-11;Transcription factor 11;transcription factor 11 (basic leucine zipper type);transcription factor HBZ17;transcription factor LCR-F1.
NCBI Gene Id4779
Protein NameQ14494
Description of TargetEndoplasmic reticulum membrane sensor NFE2L1
Uniprot IDhttps://www.uniprot.org/uniprot/Q14494
Protein Accession #NP_003195.1
Nucleotide Accession #NM_003204.2
Protein Size (# AA)772
Molecular Weight85 kDa
Write Your Own Review
You're reviewing:NFE2L1 ELISA Kit (Human) (OKEH02447)
Your Rating
We found other products you might like!