Neuropilin-2 ELISA Kit (Mouse) (OKBB00613)

Catalog No: OKBB00613

Size:96 Wells

Mouse Neuropilin-2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse Neuropilin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Neuropilin-2 has been precoated onto 96-well plates. Standards(NS0, Q23-D863) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Neuropilin-2 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1590	3425	6308	1736	3621	6738
  SD	68.37	178.1	359.6	95.48	231.7	464.9
  CV%	4.3	5.2	5.7	5.5	6.4	6.9

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse Neuropilin-2 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse Neuropilin-2 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse Neuropilin-2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, Cell lysates and tissue homogenates
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse Neuropilin-2
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q23-D863
• Description: For quantitative detection of mouse Neuropilin-2 in cell culture supernatants and cell lysates.

Target Info

• Gene Symbol: NRP2
• Gene Full Name: neuropilin 2
• Alias Symbols: MGC126574, Neuropilin 2, Neuropilin-2, Neuropilin2, NP 2, NP2, NPN 2, NPN2, NRP 2, Nrp2, NRP2_HUMAN, PRO2714, Receptor for VEGF165 and semaphorins class3, Vascular endothelial cell growth factor 165 receptor 2, Vascular endothelial growth factor 165 receptor 2, VEGF1265R2, VEGF165R2
• NCBI Gene Id: 18187
• Protein Name: Neuropilin-2
• Description of Target: NRP2 (Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.
• Uniprot ID: O35375
• Protein Accession #: NP_001070871.1
• Nucleotide Accession #: NM_001077403.1