Neuropilin-1 ELISA Kit (Mouse) (OKBB00611)

Catalog No: OKBB00611

Size:96 Wells

Mouse Neuropilin-1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse Neuropilin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Neuropilin-1 has been precoated onto 96-well plates. Standards(NS0, F22-P856) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Neuropilin-1 amount of sample captured in plate.
• ELISA Kit Range: 780 pg/ml - 50000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	7.7	19	28	8.3	25	34
  SD	0.393	1.178	1.316	0.465	1.85	1.802
  CV%	5.1	6.2	4.7	5.6	7.4	5.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse Neuropilin-1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse Neuropilin-1 Standard: 2 x 50 ng/tube
  • 100X Biotinylated Anti-Mouse Neuropilin-1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, and plasma (heparin, EDTA)
• Sensitivity: <20 pg/ml
• Specificity: Natural and recombinant mouse Neuropilin-1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: F22-P856
• Description: For quantitative detection of mouse Neuropilin-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: NRP1
• Gene Full Name: neuropilin 1
• Alias Symbols: A5 protein, BDCA4, BLOOD DENDRITIC CELL ANTIGEN 4, CD 304, CD304, DKFZp686A03134, DKFZp781F1414, Neuropilin-1, Neuropilin1, Neuropilin1 transmembrane receptor, NP1, NPN1, NRP 1, NRP, NRP1, NRP1_HUMAN, Vascular endothelial cell growth factor 165 receptor, VEGF165R
• NCBI Gene Id: 18186
• Protein Name: Neuropilin-1
• Description of Target: NRP1 (Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.
• Uniprot ID: P97333
• Protein Accession #: NP_032763.2
• Nucleotide Accession #: NM_008737.2