Neuregulin-1/NRG1-beta1 ELISA Kit (Human) (OKBB00630)

Catalog No: OKBB00630

Size:96 Wells

Human Neuregulin-1/NRG1-beta1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human Neuregulin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neuregulin-1 has been precoated onto 96-well plates. Standards(E.coli, S2-K246) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuregulin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Neuregulin-1 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	675	1308	2147	694	1382	2503
  SD	28.35	61.48	113.8	40.25	84.3	172.7
  CV%	4.2	4.7	5.3	5.8	6.1	6.9

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human Neuregulin-1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human Neuregulin-1 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human Neuregulin-1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human Neuregulin-1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: S2-K246
• Description: For quantitative detection of human Neuregulin-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: NRG1
• Gene Full Name: neuregulin 1
• Alias Symbols: Acetylcholine receptor inducing activity, Acetylcholine receptor-inducing activity, ARIA, Breast cancer cell differentiation factor p45, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, MST131, NDF, Neu differentiation factor, Neuregulin 1, Neuregulin-1, Neuregulin1, nrg1, NRG1 beta 1, NRG1_HUMAN, Pro neuregulin 1, Pro NRG1, Pro-NRG1, Sensory and motor neuron derived factor, Sensory and motor neuron-derived factor, SMDF
• NCBI Gene Id: 3084
• Protein Name: Pro-neuregulin-1, membrane-bound isoform
• Description of Target: Neuregulin 1 or NRG1 is a protein that in humans is encoded by the NRG1 gene. It is mapped to 8p12. NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). It is also essential for the normal development of the nervous system and the heart. Furthermore, it was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.
• Uniprot ID: Q02297
• Protein Accession #: NP_001153467.1
• Nucleotide Accession #: NM_001159995.1