NENF ELISA Kit (Human) (OKCD00539)

Catalog No: OKCD00539 (Formerly GWB-KBBSA8)

Size:96WELLS

Human NENF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	79-103%	94-105%	79-102%	82-96%
  EDTA Plasma (n=5)	86-95%	80-94%	90-103%	82-94%
  Heparin Plasma (n=5)	97-105%	78-90%	81-97%	80-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neudesin Neurotrophic Factor (NENF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neudesin Neurotrophic Factor (NENF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neudesin Neurotrophic Factor (NENF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neudesin Neurotrophic Factor (NENF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	101
  EDTA plasma(n=5)	83-98	94
  heparin plasma(n=5)	93-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neudesin Neurotrophic Factor (NENF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neudesin Neurotrophic Factor (NENF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NENF Microplate: 96 Wells (12 x 8 Well Strips)
  • NENF Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated NENF Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neudesin Neurotrophic Factor (NENF). No significant cross-reactivity or interference between Neudesin Neurotrophic Factor (NENF) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NENF
• Gene Full Name: neudesin neurotrophic factor
• Alias Symbols: cell growth-inhibiting protein 47;cell immortalization-related protein 2;CIR2;neudesin;neuron-derived neurotrophic factor;Protein GIG47;SCIRP10;SCIRP10-related protein;secreted protein of unknown function;Spinal cord injury related protein 10;SPUF.
• NCBI Gene Id: 29937
• Protein Name: Neudesin
• Description of Target: Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity). Plays a role in the human tumorigenesis (PubMed:22748190).
• Uniprot ID: Q9UMX5
• Protein Accession #: NP_037481
• Nucleotide Accession #: NM_013349.4
• Protein Size (# AA): 172