Catalog No: OKCD01073 (Formerly GWB-KBBTM4)
Size:96WELLS
Price: $750.00
SKU
OKCD01073
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Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEL Like Protein 2 (NELL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NEL Like Protein 2 (NELL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEL Like Protein 2 (NELL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NEL Like Protein 2 (NELL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEL Like Protein 2 (NELL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEL Like Protein 2 (NELL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NELL2 Microplate96 Wells (12 x 8 Well Strips)
NELL2 Lyophilized Standard2 x 20 ng
100X Biotinylated NELL2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.115 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of NEL Like Protein 2 (NELL2). No significant cross-reactivity or interference between NEL Like Protein 2 (NELL2) and analogues was observed.
Publications

NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth. Cell Rep. 36, 109254 (2021)

Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Description
Gene SymbolNELL2
Gene Full Nameneural EGFL like 2
Alias SymbolsNEL-like protein 2;NEL-related protein 2;neural epidermal growth factor-like 2;NRP2;protein kinase C-binding protein NELL2.
NCBI Gene Id4753
Protein NameProtein kinase C-binding protein NELL2
Description of TargetRequired for neuron survival through the modulation of MAPK pathways (By similarity). Involved in the regulation of hypothalamic GNRH secretion and the control of puberty (By similarity).
Uniprot IDQ99435
Protein Accession #NP_001138579
Nucleotide Accession #NM_001145107.1
Protein Size (# AA)816
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