Nefl High Sensitivity ELISA Kit (Mouse) (OKCD01381)

Catalog No: OKCD01381

Size:96WELLS

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	83-97%	93-103%	94-102%
  EDTA Plasma (n=5)	82-103%	81-96%	79-95%	82-102%
  Heparin Plasma (n=5)	94-102%	87-96%	92-101%	90-97%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Neurofilament, Light Polypeptide (NEFL) and unlabeled High Sensitive Neurofilament, Light Polypeptide (NEFL) (Standards or samples) with the pre-coated antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the sample.
• ELISA Kit Range: 4.94-400pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	81-96	91
  EDTA Plasma (n=5)	94-105	102
  Heparin Plasma (n=5)	78-98	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Nefl Microplate: 96 Wells (12 x 8 Well Strips)
  • Nefl Lyophilized Standard: 2
  • 100X Biotinylated Nefl Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, cerebrospinal fluid and other biological fluids
• Sensitivity: 1.95 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL). No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Nefl
• Gene Full Name: neurofilament, light polypeptide
• Alias Symbols: 68 kDa neurofilament protein;AI847934;CMT2E;N;neurofilament light polypeptide;neurofilament triplet L protein;NF;NF6;NF68;Nfl;NF-L.
• NCBI Gene Id: 18039
• Protein Name: Neurofilament light polypeptide
• Description of Target: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
• Uniprot ID: P08551
• Protein Accession #: NP_035040.1
• Nucleotide Accession #: NM_010910.1