NDRG2 ELISA Kit (Rat) (OKCD01072)

Catalog No: OKCD01072 (Formerly GWB-KBBBB4)

Size:96WELLS

Rat Ndrg2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-88%	86-95%	79-101%	82-102%
  EDTA plasma(n=5)	78-101%	82-93%	79-92%	94-102%
  heparin plasma(n=5)	78-103%	78-103%	86-104%	82-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-myc Downstream Regulated Gene 2 (NDRG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-myc Downstream Regulated Gene 2 (NDRG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-myc Downstream Regulated Gene 2 (NDRG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of N-myc Downstream Regulated Gene 2 (NDRG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	102
  EDTA plasma(n=5)	82-89	85
  heparin plasma(n=5)	83-94	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-myc Downstream Regulated Gene 2 (NDRG2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-myc Downstream Regulated Gene 2 (NDRG2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ndrg2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Ndrg2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Ndrg2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.141 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of N-myc Downstream Regulated Gene 2 (NDRG2). No significant cross-reactivity or interference between N-myc Downstream Regulated Gene 2 (NDRG2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Ndrg2
• Gene Full Name: NDRG family member 2
• Alias Symbols: antidepressant-related protein ADRG123;NDRG1-related protein;N-myc downstream regulated gene 2;N-myc downstream-regulated gene 2 protein;protein NDRG2.
• NCBI Gene Id: 171114
• Protein Name: Protein NDRG2
• Description of Target: Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor (By similarity). May be involved in dendritic cell and neuron differentiation.
• Uniprot ID: Q8VBU2
• Protein Accession #: NP_001257791
• Nucleotide Accession #: NM_001270862.1
• Protein Size (# AA): 371