NCSTN Antibody (OAPB00467)

Catalog No: OAPB00467

Size:100UG

Click here to learn more about Aviva's By-Request Conjugation Service.

Western blot analysis of Nicastrin in mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. Nicastrin Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: Nicastrin antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Nicastrin antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human Nicastrin. The immunogen is located within amino acids 630 - 680 of Nicastrin.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-NCSTN (OAPB00467) antibody is Catalog # OEPB02577
• Application Info: Nicastrin antibody can be used for detection of Nicastrin by Western blot at 0.5 - 1 ug/ml. (Optimal dilution should be determined by user)
• Reference: Weihofen A and Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 2003; 13:71-8.; Periz G and Fortini ME. Functional reconstitution of g-secretase through coordinated expression of presenilin, Nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.;Selkoe DJ. The cell biology of b-amyloid precursor protein and presenilin in Alzheimer’s disease. Trends Cell Biol. 1998; 8:447-53.;Nguyen V, Hawkins C, Bergeron C, et al. Loss of nicastrin elicits an apoptotic phenotype in mouse embryos. Brain Res. 2006; 1086:76-84.

Target Info

• Gene Symbol: NCSTN
• Gene Full Name: nicastrin
• Alias Symbols: anterior pharynx-defective 2;ATAG1874;nicastrin.
• NCBI Gene Id: 23385
• Protein Name: Nicastrin|nicastrin isoform 1 precursor [Homo sapiens]
• Description of Target: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:10993067, PubMed:12679784, PubMed:25043039, PubMed:26280335). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
• Uniprot ID: Q92542
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_056146
• Nucleotide Accession #: NM_001290184.1; NM_001290186.1; NM_015331.2