NCR1 Antibody (OASA00280)

Catalog No: OASA00280 (Formerly GWB-220A98)

Size:500UG

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD335. A small positive peak representing NK cells can be identified

Product Info

• Predicted Species Reactivity: Bovine
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: AKS1
• Isotype: IgG1
• Host: Mouse
• Conjugation: LE: Low Endotoxin
• Application: Flow cytometry|Functional assay|Immunofluorescence|Western blot
• Additional Information: Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the NS-0 myeloma cell line.
  Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  Preservative Stabilisers: None present
  Approx Protein Conc: IgG concentration 1.0 mg/ml
  Buffer Solutions: Phosphate buffered saline pH7.4
  Endotoxin Level: < 0.01EU/ug
• Reconstitution and Storage: -20°C
• Immunogen: Fusion protein consisting of the extracellular region of bovine CD335.
• Purification: Protein A Purified
• Predicted Homology Based on Immunogen Sequence: Cow
• Concentration: 1 mg/ml
• Specificity: CD335
• Application Info: Flow Cytometry:    1/5 - 1/200
  following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.

  	Yes	No	Not Determined	Suggested Dilution
  Flow Cytometry				1/5 - 1/200
  Immunohistology - Frozen
  Immunohistology - Paraffin
  Immunohistology - Resin
  ELISA
  Immunoprecipitation
  Western Blotting
  Functional Assays

  Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system to a concentration equivalent to their test reagent.
• Application Data: Application #1: Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD335 . A small positive peak representing NK cells can be identified
• Reference: 1. Storset, A.K. et al. (2003) Natural killer cell receptors in cattle: a bovine killer cell immunoglobulin-like receptor multigene family contains members with divergent signaling motifs. Eur. J. Immunol. 33:980-990.
  2. Storset, A.K. et al. (2004) NKp46 defines a subset of bovine leukocytes with natural killer characteristics. Eur. J. Immunol. 34:669-676.
  3. Kulberg, S. et al. (2004) Reference values for relative numbers of natural killer cells in cattle blood. Dev. Comp. Immunol. 28:941-948.
  4. Sivori, S. et al. (1997) p46, a novel natural killer cell-specific surface molecule that mediates cell activation. J. Exp. Med. 186:1129-1136.
  

Target Info

• Gene Symbol: NCR1
• Alias Symbols: NKP46
• NCBI Gene Id: 369024
• Protein Name: Natural cytotoxicity triggering receptor 1
• Description of Target: MOUSE ANTI BOVINE CD335:Low Endotoxin
  
• Uniprot ID: Q863H2
• Protein Accession #: NP_899209.1
• Protein Size (# AA): 308