NCF1 ELISA Kit (Human) (OKCD00880)

Catalog No: OKCD00880 (Formerly GWB-KBBAE1)

Size:96WELLS

Human NCF1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Cytosolic Factor 1 (NCF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neutrophil Cytosolic Factor 1 (NCF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Cytosolic Factor 1 (NCF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Cytosolic Factor 1 (NCF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NCF1 Microplate: 96 Wells (12 x 8 Well Strips)
  • NCF1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated NCF1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neutrophil Cytosolic Factor 1 (NCF1). No significant cross-reactivity or interference between Neutrophil Cytosolic Factor 1 (NCF1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NCF1
• Gene Full Name: neutrophil cytosolic factor 1
• Alias Symbols: 47 kDa autosomal chronic granulomatous disease protein;47 kDa neutrophil oxidase factor;CGD1;NADPH oxidase organizer 2;NCF-1;NCF1A;NCF-47K;neutrophil cytosol factor 1;neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1);neutrophil NADPH oxidase factor 1;nox organizer 2;NOXO2;nox-organizing protein 2;p47phox;p47-phox;SH3 and PX domain-containing protein 1A;SH3PXD1A.
• NCBI Gene Id: 653361
• Protein Name: Neutrophil cytosol factor 1
• Description of Target: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
• Uniprot ID: P14598
• Protein Accession #: NP_000256
• Nucleotide Accession #: NM_000265.5
• Protein Size (# AA): 390