NBR1 ELISA Kit (Human) (OKEH06788)

Catalog No: OKEH06788

Size:96T

Human NBR1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology NBR1 ELISA Kit (Human) (OKEH06788) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NBR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NBR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NBR1 captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into serum = 96%
• ELISA Kit Reproducibility: Intra-Assay Reproducibility CV% < 4.8% (n=20)< br>Inter-Assay Reproducibility CV% < 8.6% (n=24)
• ELISA Kit Component:
  • SAAL1 Microplate: 96 Wells (12 x 8 Well strips)
  • SAAL1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SAAL1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.082ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NBR1
• Gene Full Name: NBR1 autophagy cargo receptor
• Alias Symbols: 1A1-3B;B-box protein;cell migration-inducing gene 19 protein;IAI3B;M17S2;Membrane component chromosome 17 surface marker 2;membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125);MIG19;migration-inducing protein 19;neighbor of BRCA1 gene 1;Neighbor of BRCA1 gene 1 protein;next to BRCA1 gene 1 protein;Protein 1A1-3B.
• NCBI Gene Id: 4077
• Protein Name: Q14596
• Description of Target: Next to BRCA1 gene 1 protein
• Uniprot ID: https://www.uniprot.org/uniprot/Q14596
• Protein Accession #: NP_001278500
• Nucleotide Accession #: NM_001291571