Catalog No: OKEH01118
Size:96T
Price: $525.00
SKU
OKEH01118
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NARG2 ELISA Kit (Human) (OKEH01118) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NARG2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NARG2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NARG2 captured in well.
ELISA Kit Range15.6-1000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
NARG2 Microplate96 Wells (12 x 8 Well strips)
NARG2 Lyophilized Standard2
100X Biotinylated NARG2 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human NMDA receptor-regulated protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolICE2
Gene Full Nameinteractor of little elongation complex ELL subunit 2
Alias SymbolsBRCC1;breast cancer cell 1;interactor of little elongator complex ELL subunit 2;little elongation complex subunit 2;NARG2;NMDA receptor regulated 2;NMDA receptor-regulated gene 2;NMDA receptor-regulated protein 2.
NCBI Gene Id79664
Protein NameQ659A1
Description of TargetLittle elongation complex subunit 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q659A1
Protein Accession #NP_001018099.1
Nucleotide Accession #NM_001018089.2
Protein Size (# AA)982
Molecular Weight110 kDa
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