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Catalog No: OKEH04362
Size:96T
Price: $525.00
SKU
OKEH04362
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Nampt ELISA Kit (Rat) (OKEH04362) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nampt has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Nampt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nampt captured in the well.
ELISA Kit Range78-5000pg/ml
ELISA Kit Component
ComponentAmount
Anti-Nampt Microplate96 Wells (12 x 8 Well Strips)
Nampt Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Nampt Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.7 pg/mL
SpecificityNatural and recombinant Rat Nicotinamide phosphoribosyltransferase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNampt
Gene Full Namenicotinamide phosphoribosyltransferase
Alias SymbolsNAmPRTase;nicotinamide phosphoribosyltransferase;Pbef;Pbef1;pre-B-cell colony enhancing factor 1;pre-B-cell colony-enhancing factor 1 homolog;visfatin.
NCBI Gene Id297508
Protein NameQ80Z29
Description of TargetNicotinamide phosphoribosyltransferase
Uniprot IDhttps://www.uniprot.org/uniprot/Q80Z29
Protein Accession #NP_808789.1
Nucleotide Accession #NM_177928.3
Protein Size (# AA)491
Molecular Weight55 KDa
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