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Catalog No: OKCD00512 (Formerly GWB-KBBFR1)
Size:96WELLS
Price: $675.00
SKU
OKCD00512
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Datasheets/ManualsPrintable datasheet for MYPN ELISA Kit (Human) (OKCD00512)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myopalladin (MYPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myopalladin (MYPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myopalladin (MYPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myopalladin (MYPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myopalladin (MYPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myopalladin (MYPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MYPN Microplate96 Wells (12 x 8 Well Strips)
MYPN Lyophilized Standard2 x 10 ng
100X Biotinylated MYPN Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.066 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myopalladin (MYPN). No significant cross-reactivity or interference between Myopalladin (MYPN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMYPN
Gene Full Namemyopalladin
Alias Symbols145 kDa sarcomeric protein;CMD1DD;CMH22;MYOP;myopalladin;NEM11;RCM4;sarcomeric protein myopalladin, 145 kDa (MYOP).
NCBI Gene Id84665
Protein NameMyopalladin
Description of TargetComponent of the sarcomere that tethers together nebulin (skeletal muscle) and nebulette (cardiac muscle) to alpha-actinin, at the Z lines.
Uniprot IDQ86TC9
Protein Accession #NP_001243196
Nucleotide Accession #NM_001256267.1
Protein Size (# AA)1320
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