MYL2 ELISA Kit (Human) (OKDD00413)

Catalog No: OKDD00413 (Formerly GWB-KBBVV9)

Size:96 Wells

Human MYL2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology MYL2 ELISA Kit (Human) (OKDD00413) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MYL2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYL2 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MYL2 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MYL2 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MYL2 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-MYL2 Microplate: 96 Wells (12 x 8 Well Strips)
  • MYL2 Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X MYL2 Biotinylated Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 ÂuL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of MYL2. No significant cross-reactivity or interference between MYL2 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MYL2
• Gene Full Name: myosin light chain 2
• Alias Symbols: cardiac myosin light chain 2;cardiac ventricular myosin light chain 2;CMH10;MFM12;MLC2;MLC-2;MLC-2s/v;MLC-2v;Myosin light chain 2, slow skeletal/ventricular muscle isoform;myosin regulatory light chain 2, ventricular/cardiac muscle isoform;myosin, light chain 2, regulatory, cardiac, slow;myosin, light polypeptide 2, regulatory, cardiac, slow;regulatory light chain of myosin;RLC of myosin;slow cardiac myosin regulatory light chain 2;truncated myosin light chain 2;ventricular myosin light chain 2.
• NCBI Gene Id: 4633
• Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• Description of Target: Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
• Uniprot ID: P10916
• Protein Accession #: NP_000423.2
• Nucleotide Accession #: NM_000432.3
• Protein Size (# AA): 166
• Molecular Weight: 19 kDa