MYH7 ELISA Kit (Rat) (OKEH03043)

Catalog No: OKEH03043

Size:96T

Rat MYH7 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MYH7 ELISA Kit (Rat) (OKEH03043) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Myh7 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Myh7 and incubated. The Myh7 found in the sample or standards competes with the biotinylated Myh7 for limited binding sites on the immobilized anti-rat Myh7 antibody. Excess unbound biotinylated Myh7 and sample or standard Myh7 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Myh7 captured in the well and inversely proportional to the amount of Myh7 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into serum: 93%
• ELISA Kit Reproducibility: Mean Intra-Assay CV: ≤4.8% (n = 20)
  Mean Inter-Assay CV: ≤7.9% (n = 24)
• ELISA Kit Component:
  • Anti-Myh7 Microplate: 96 Wells (12 x 8 Well strips)
  • Myh7 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Myh7-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.182 ng/mL
• Specificity: Natural and recombinant Rat Myosin-7
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Myh7
• Gene Full Name: myosin heavy chain 7
• Alias Symbols: beta myosin heavy chain;Bmyo;Myhcb;myHC-beta;myHC-slow;Myosin heavy chain 7;myosin heavy chain polypeptide 7 cardiac muscle fetal;Myosin heavy chain slow isoform;Myosin heavy chain, cardiac muscle beta isoform;myosin heavy chain, cardiac muscle, fetal;myosin heavy chain, polypeptide 7;myosin, heavy chain 7, cardiac muscle, beta;myosin, heavy polypeptide 7, cardiac muscle, beta;myosin-7.
• NCBI Gene Id: 29557
• Protein Name: P02564
• Description of Target: Myosin-7
• Uniprot ID: https://www.uniprot.org/uniprot/P02564
• Protein Accession #: NP_058936.1
• Nucleotide Accession #: NM_017240.2
• Protein Size (# AA): 1935
• Molecular Weight: 223 kDa