Catalog No: OKDD00714 (Formerly GWB-KBBOQ4)
Size:96 Wells
Price: $850.00
SKU
OKDD00714
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Datasheets/ManualsPrintable datasheet for MYH6 ELISA Kit (Rat) (OKDD00714)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology MYH6 ELISA Kit (Rat) (OKDD00714) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for MYH6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYH6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MYH6 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-MYH6 Microplate96 Wells (12 x 8 Well Strips)
MYH6 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X MYH6 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates
Sensitivity<0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of MYH6. No significant cross-reactivity or interference between MYH6 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolMyh6
Gene Full Namemyosin heavy chain 6
Alias SymbolsMyh7;Myhca;myHC-alpha;MyHC-beta;MyHC-slow;Myosin heavy chain 6;Myosin heavy chain 7;myosin heavy chain polypeptide 6 cardiac muscle adult;Myosin heavy chain, cardiac muscle alpha isoform;myosin heavy chain, polypeptide 6, cardiac muscle, alpha;myosin, heavy chain 6, cardiac muscle, alpha;myosin, heavy polypeptide 6, cardiac muscle, alpha;myosin-6;Myosin-7.
NCBI Gene Id29556
Protein NameMyosin-6
Description of TargetMuscle contraction.
Uniprot IDP02563
Protein Accession #NP_058935.2
Nucleotide Accession #NM_017239.2
Protein Size (# AA)1938
Molecular Weight224 kDa
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