MYH11 ELISA Kit (Human) (OKEH04826)

Catalog No: OKEH04826

Size:96T

Human MYH11 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology MYH11 ELISA Kit (Human) (OKEH04826) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYH11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYH11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYH11 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • MYH11 Microplate: 96 Wells (12 x 8 Well Strips)
  • MYH11 Standard: 2
  • Sample Diluent: 1 x 20 mL
  • MYH11 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.25 ng/mL
• Specificity: Natural and recombinant Human Myosin-11
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MYH11
• Gene Full Name: myosin heavy chain 11
• Alias Symbols: AAT4;epididymis secretory sperm binding protein;FAA4;Myosin heavy chain 11;Myosin heavy chain, smooth muscle isoform;myosin, heavy chain 11, smooth muscle;myosin, heavy polypeptide 11, smooth muscle;myosin-11;SMHC;SMMHC;VSCM2.
• NCBI Gene Id: 4629
• Protein Name: P35749
• Description of Target: Myosin-11
• Uniprot ID: https://www.uniprot.org/uniprot/P35749
• Protein Accession #: NP_001035202.1
• Nucleotide Accession #: NM_001040113.1
• Protein Size (# AA): 1972
• Molecular Weight: 227 kDa