MYC ELISA Kit (Human) (OKEH04573)

Catalog No: OKEH04573

Size:96T

Human MYC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology MYC ELISA Kit (Human) (OKEH04573) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for MYC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYC captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-MYC Microplate: 96 Wells (12 x 8 Well Strips)
  • MYC Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • MYC Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 9.4 pg/mL
• Specificity: Natural and recombinant Human Myc proto-oncogene protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MYC
• Gene Full Name: MYC proto-oncogene, bHLH transcription factor
• Alias Symbols: avian myelocytomatosis viral oncogene homolog;bHLHe39;class E basic helix-loop-helix protein 39;c-Myc;MRTL;myc proto-oncogene protein;MYCC;myc-related translation/localization regulatory factor;proto-oncogene c-Myc;transcription factor p64;v-myc avian myelocytomatosis viral oncogene homolog;v-myc myelocytomatosis viral oncogene homolog.
• NCBI Gene Id: 4609
• Protein Name: P01106
• Description of Target: Myc proto-oncogene protein
• Uniprot ID: https://www.uniprot.org/uniprot/P01106
• Protein Accession #: NP_002458.2
• Nucleotide Accession #: NM_002467.4
• Protein Size (# AA): 439
• Molecular Weight: 49 KDa