Mutated Citrullinated Vimentin Antibody (Human) ELISA Kit (OKCA00429)

Catalog No: OKCA00429

Size:96 Wells

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Mutated Citrullinated Vimentin Antibody (Human) ELISA Kit (OKCA00429) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Mutated Citrullinated Vimentin antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanAntibody is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mutated Citrullinated Vimentin Antibody captured in well.
• ELISA Kit Reproducibility: Three different samples of known Mutated Citrullinated Vimentin Antibody concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
  Intra-Assay CV: <15%
  Inter-Assay CV: <15%
• ELISA Kit Component:
  • Mutated Citrullinated Vimentin Antibody Microplate: 96 Wells (12 x 8 Well strips)
  • Standard: 5 x 1 mL
  • Negative Control: 1 x 0.8 mL
  • Positive Control: 1 x 0.8 mL
  • Anti-Human Antibody HRP-Conjugate: 1 x 10 mL
  • Sample Diluent: 1 x 20 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Substrate A: 1 x 5 mL
  • Substrate B: 1 x 5 mL
  • Stop Solution: 1 x 5 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum and Plasma
• Assay Info: Assay Methodology: Qualitative Reverse Capture ELISA

Target Info

• Alias Symbols: MCV
• Protein Name: Mutated Citrullinated Vimentin Antibody
• Description of Target: Mutated Citrullinated Vimentin Antibody