MUSK ELISA Kit (Mouse) (OKCD02738)

Catalog No: OKCD02738

Size:96WELLS

Mouse MUSK ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MUSK Microplate: 96 Wells (12 x 8 Well strips)
  • MUSK Lyophilized Standard: 2
  • 100X Biotinylated MUSK Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.061 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). No significant cross-reactivity or interference between Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Musk
• Gene Full Name: muscle, skeletal, receptor tyrosine kinase
• Alias Symbols: Mdk4;Mlk;muscle localized kinase 2;muscle, skeletal receptor tyrosine-protein kinase;muscle-specific kinase receptor;muscle-specific tyrosine-protein kinase receptor;Ns;Nsk1;Nsk2;Nsk3.
• NCBI Gene Id: 18198
• Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Description of Target: Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.
• Uniprot ID: Q61006
• Protein Accession #: NP_001032205.1
• Nucleotide Accession #: NM_001037128.1
• Protein Size (# AA): 868