Catalog No: OKCD00811 (Formerly GWB-KBBOS1)
Size:96WELLS
Price: $650.00
SKU
OKCD00811
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Datasheets/ManualsPrintable datasheet for MUC5B ELISA Kit (Rat) (OKCD00811)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype B (MUC5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype B (MUC5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 5 Subtype B (MUC5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mucin 5 Subtype B (MUC5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype B (MUC5B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype B (MUC5B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Muc5b Microplate96 Wells (12 x 8 Well Strips)
Muc5b Lyophilized Standard2 x 20 ng
100X Biotinylated Muc5b Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.119 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mucin 5 Subtype B (MUC5B). No significant cross-reactivity or interference between Mucin 5 Subtype B (MUC5B) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMUC5B
Gene Full Namemucin 5B, oligomeric mucus/gel-forming
Alias SymbolsRGD1561983
NCBI Gene Id309114
Protein NameMucin 5B, oligomeric mucus/gel-forming
Description of TargetMucin 5B, oligomeric mucus/gel-forming
Uniprot IDD3ZAU0
Protein Accession #XP_006230670
Nucleotide Accession #XM_006230608.2
Protein Size (# AA)4156
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