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Catalog No: OKDD00616 (Formerly GWB-KBBDN6)
Size:96 Wells
Price: $750.00
SKU
OKDD00616
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Datasheets/ManualsPrintable datasheet for MUC5AC ELISA Kit (Mouse) (OKDD00616)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology MUC5AC ELISA Kit (Mouse) (OKDD00616) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for MUC5AC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MUC5AC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MUC5AC captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-MUC5AC Microplate96 Wells (12 x 8 Well Strips)
MUC5AC Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X MUC5AC Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates
Sensitivity<0.053 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolMuc5ac
Gene Full Namemucin 5, subtypes A and C, tracheobronchial/gastric
Alias Symbols2210005L13Rik;gastric mucin;MGM;mucin-5AC.
NCBI Gene Id17833
Protein NameMucin 5, subtypes A and C, tracheobronchial/gastric
Uniprot IDE9PWB6
Protein Accession #NP_034974.1
Nucleotide Accession #NM_010844.1
Protein Size (# AA)2751
Molecular Weight299 kDa
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