MUC1 Antibody - middle region (ARP41444_P050)

Catalog No: ARP41444_P050

Sample Type:
Pig stomach
Primary Antibody Dilution:
1:200
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
Brown: MUC1 Blue: Nucleus
Gene Name:
MUC1
Submitted by:
Dr. Sara Linden, University of Gothenburg

Sample Type:
Human stomach
Primary Antibody Dilution:
1:200
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
Brown: MUC1 Blue: Nucleus
Gene Name:
MUC1
Submitted by:
Dr. Sara Linden, University of Gothenburg

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The protein may be modified by glycosylation.

Product Info

• Tested Species Reactivity: Human, Pig
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MUC1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 79%; Dog: 79%; Goat: 79%; Human: 100%; Mouse: 86%; Pig: 83%; Rabbit: 86%; Rat: 93%
• Peptide Sequence: Synthetic peptide located within the following region: ASSTPGGEKETSATQRSSVPSSTEKNAFNSSLEDPSTDYYQELQRDISEM
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-MUC1 (ARP41444_P050) antibody is Catalog # AAP41444 (Previous Catalog # AAPP24181)
• Sample Type Confirmation:

  MUC1 is strongly supported by BioGPS gene expression data to be expressed in HeLa

• Reference: Creaney,J., (2008) Br. J. Cancer 98 (9), 1562-1569

Target Info

• Gene Symbol: MUC1
• Gene Full Name: Mucin 1, cell surface associated
• Alias Symbols: EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADTKD2, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC
• NCBI Gene Id: 4582
• Protein Name: Mucin 1, cell surface associated EMBL CAI95072.1
• Description of Target: MUC1 is a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.
• Uniprot ID: Q7Z550
• Protein Accession #: NP_001037857
• Nucleotide Accession #: NM_001044392
• Protein Size (# AA): 1255
• Molecular Weight: 122 kDa
• Protein Interactions: UBC; PPARG; IPO5; NUP62; EGFR; ABL1; SRC; JUN; TP53; TBP; IKBKG; TNFRSF1A; IKBKB; GRB2; ESR1; GALNT15; GALNT12; GALNT10; OSGEP; GSK3B; GALNT2; GALNT1; CTNND1; SIGLEC1; ERBB4; ERBB3; SOS1; PRKCD; ZAP70; APC; JUP; LYN; ERBB2; LCK; CTNNB1; GALNT4; BAX; CDKN1