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Catalog No: OKCD01182 (Formerly GWB-KBBYB2)
Size:96WELLS
Price: $700.00
SKU
OKCD01182
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Datasheets/ManualsPrintable datasheet for MTPN ELISA Kit (Mouse) (OKCD01182)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)88-96%96-103%99-105%80-97%
EDTA plasma(n=5)79-92%78-95%82-98%88-95%
heparin plasma(n=5)91-99%89-101%89-101%78-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myotrophin (MTPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myotrophin (MTPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myotrophin (MTPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myotrophin (MTPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range6.25-400pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)99-105102
EDTA plasma(n=5)92-10499
heparin plasma(n=5)96-104101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myotrophin (MTPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myotrophin (MTPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Mtpn Microplate96 Wells (12 x 8 Well Strips)
Mtpn Lyophilized Standard2 x 400 pg
100X Biotinylated Mtpn Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 2.67 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myotrophin (MTPN). No significant cross-reactivity or interference between Myotrophin (MTPN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMtpn
Gene Full Namemyotrophin
Alias Symbols5033418D15Rik;Gcd;Gcdp;granule cell differentiation protein;myotrophin;protein V-1;V;V1.
NCBI Gene Id14489
Protein NameMyotrophin
Description of TargetPromotes dimerization of NF-kappa-B subunits and regulates NF-kappa-B transcription factor activity. Promotes growth of cardiomyocytes, but not cardiomyocyte proliferation. Promotes cardiac muscle hypertrophy (By similarity). Plays a role in the regulation of the growth of actin filaments. Inhibits the activity of the F-actin-capping protein complex formed by the CAPZA1 and CAPZB heterodimer.
Uniprot IDP62774
Protein Accession #NP_032124
Nucleotide Accession #NM_008098.4
Protein Size (# AA)118
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