MTOR ELISA Kit (Human) : 96 Wells (OKEH02402)

Catalog No: OKEH02402

Size:96T

Human MTOR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MTOR ELISA Kit (Human) (OKEH02402) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MTOR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MTOR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MTOR captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 9.2% (n = 20)
• ELISA Kit Component:
  • MTOR Microplate: 96 Wells (12 x 8 Well strips)
  • MTOR Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated C9 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 39 pg/mL
• Specificity: Natural and recombinant Human Serine/threonine-protein kinase mTOR
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MTOR
• Gene Full Name: mechanistic target of rapamycin kinase
• Alias Symbols: FK506 binding protein 12-rapamycin associated protein 2;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;FKBP12-rapamycin complex-associated protein 1;FKBP-rapamycin associated protein;FRAP;FRAP1;FRAP2;mammalian target of rapamycin;Mechanistic target of rapamycin;mechanistic target of rapamycin (serine/threonine kinase);RAFT1;rapamycin and FKBP12 target 1;rapamycin associated protein FRAP2;rapamycin target protein 1;RAPT1;serine/threonine-protein kinase mTOR;SKS.
• NCBI Gene Id: 2475
• Protein Name: P42345
• Description of Target: Serine/threonine-protein kinase mTOR
• Uniprot ID: https://www.uniprot.org/uniprot/P42345
• Protein Accession #: NP_004949.1
• Nucleotide Accession #: NM_004958.3
• Protein Size (# AA): 2549
• Molecular Weight: 289 kDa