MTHFD1L ELISA Kit (Bovine) (OKEH03924)

Catalog No: OKEH03924

Size:96T

Bovine MTHFD1L ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MTHFD1L ELISA Kit (Bovine) (OKEH03924) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MTHFD1L has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MTHFD1L is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MTHFD1L captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Component:
  • MTHFD1L Microplate: 96 Wells (12 x 8 Well strips)
  • MTHFD1L Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated MTHFD1L Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.089 ng/mL
• Specificity: Natural and recombinant Bovine Monofunctional C1-tetrahydrofolate synthase, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MTHFD1L
• Gene Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
• Alias Symbols: formyltetrahydrofolate synthetase;monofunctional C1-tetrahydrofolate synthase, mitochondrial.
• NCBI Gene Id: 534296
• Protein Name: Q0VCR7
• Description of Target: Monofunctional C1-tetrahydrofolate synthase, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/Q0VCR7
• Protein Accession #: NP_001069486.1
• Nucleotide Accession #: NM_001076018.2
• Protein Size (# AA): 975
• Molecular Weight: 975 kDa