MTAP2 ELISA Kit (Mouse) (OKCD02711)

Catalog No: OKCD02711

Size:96WELLS

Mouse MTAP2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-99%	85-103%	97-104%	80-96%
  EDTA Plasma (n=5)	78-104%	97-104%	80-96%	88-95%
  Heparin Plasma (n=5)	79-89%	86-94%	78-92%	93-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 2 (MAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 2 (MAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 2 (MAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Microtubule Associated Protein 2 (MAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-104	90
  EDTA Plasma (n=5)	85-98	89
  Heparin Plasma(n=5)	83-97	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein 2 (MAP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein 2 (MAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MTAP2 Microplate: 96 Wells (12 x 8 Well strips)
  • MTAP2 Lyophilized Standard: 2
  • 100X Biotinylated MTAP2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein 2 (MAP2). No significant cross-reactivity or interference between Microtubule Associated Protein 2 (MAP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for MAP2 ELISA kit (Mouse) on CiteAb

Target Info

• Gene Symbol: Map2
• Gene Full Name: microtubule-associated protein 2
• Alias Symbols: G1-397-34;MAP;MAP-2;microtubule-associated protein 2;Mta;Mtap2;Mtap-2;re;repro4.
• NCBI Gene Id: 17756
• Protein Name: Microtubule-associated protein 2
• Description of Target: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
• Uniprot ID: P20357
• Protein Accession #: NP_001297563.1
• Nucleotide Accession #: NM_001310634.1
• Protein Size (# AA): 1828