MTAP ELISA Kit (Human) (OKCD00486)

Catalog No: OKCD00486 (Formerly GWB-KBBRJ1)

Size:96WELLS

Human MTAP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylthioadenosine Phosphorylase (MTAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylthioadenosine Phosphorylase (MTAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylthioadenosine Phosphorylase (MTAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Methylthioadenosine Phosphorylase (MTAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methylthioadenosine Phosphorylase (MTAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methylthioadenosine Phosphorylase (MTAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MTAP Microplate: 96 Wells (12 x 8 Well Strips)
  • MTAP Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated MTAP Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.063 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Methylthioadenosine Phosphorylase (MTAP). No significant cross-reactivity or interference between Methylthioadenosine Phosphorylase (MTAP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MTAP
• Gene Full Name: methylthioadenosine phosphorylase
• Alias Symbols: 5'-methylthioadenosine phosphorylase;BDMF;c86fus;DMSFH;DMSMFH;epididymis luminal protein 249;epididymis secretory sperm binding protein;HEL-249;LGMBF;MeSAdo phosphorylase;MSAP;MTA phosphorylase;MTAPase;S-methyl-5'-thioadenosine phosphorylase.
• NCBI Gene Id: 4507
• Protein Name: S-methyl-5'-thioadenosine phosphorylase
• Description of Target: Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
• Uniprot ID: Q13126
• Protein Accession #: NP_002442
• Nucleotide Accession #: NM_002451.3
• Protein Size (# AA): 283