MTA2 ELISA Kit (Human) (OKCD01214)

Catalog No: OKCD01214 (Formerly GWB-KBBIG6)

Size:96WELLS

Human MTA2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	90-104%	93-101%	95-103%	80-99%
  EDTA plasma(n=5)	85-97%	95-102%	94-102%	78-92%
  heparin plasma(n=5)	79-97%	82-104%	89-103%	81-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metastasis Associated Protein 2 (MTA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metastasis Associated Protein 2 (MTA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metastasis Associated Protein 2 (MTA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Metastasis Associated Protein 2 (MTA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	87-98	95
  EDTA plasma(n=5)	98-105	102
  heparin plasma(n=5)	82-95	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Metastasis Associated Protein 2 (MTA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Metastasis Associated Protein 2 (MTA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MTA2 Microplate: 96 Wells (12 x 8 Well Strips)
  • MTA2 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated MTA2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 28 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Metastasis Associated Protein 2 (MTA2). No significant cross-reactivity or interference between Metastasis Associated Protein 2 (MTA2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MTA2
• Gene Full Name: metastasis associated 1 family member 2
• Alias Symbols: metastasis -associated gene 1-like 1;metastasis associated gene family, member 2;metastasis-associated 1-like 1;metastasis-associated protein 2;metastasis-associated protein MTA2;MTA1L1;MTA1-L1 protein;p53 target protein in deacetylase complex;PID.
• NCBI Gene Id: 9219
• Protein Name: Metastasis-associated protein MTA2
• Description of Target: May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.
• Uniprot ID: O94776
• Protein Accession #: NP_001094210
• Nucleotide Accession #: NM_001100740.1
• Protein Size (# AA): 668