MSTN ELISA Kit (Horse) (OKCA01065)

Catalog No: OKCA01065

Size:96 Wells

Horse MSTN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Horse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	90	83-94
  1:2	96	89-100
  1:4	97	89-102
  1:8	103	98-107

• ELISA Kit Principle: Aviva Systems Biology MSTN ELISA Kit (Horse) (OKCA01065) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MSTN has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MSTN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MSTN captured in the well.
• ELISA Kit Range: 6.25 - 400 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	100	90-104
  EDTA Plasma (n=4)	89	83-95

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01065 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01065 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • MSTN Microplate: 96 Wells (12 x 8 Well strips)
  • MSTN Lyophilized Standard: 2 x 400 ng
  • 100X Biotinylated MSTN Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 1.56 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: MSTN
• Gene Full Name: Growth/differentiation factor 8
• Alias Symbols: GDF8, growth differentiation factor 8
• NCBI Gene Id: 100033832
• Protein Name: Growth/differentiation factor 8
• Description of Target: Acts specifically as a negative regulator of skeletal muscle growth.
• Uniprot ID: Q9GM97
• Protein Accession #: NP_001075286.1
• Nucleotide Accession #: NM_001081817.1