MSRA ELISA Kit (Human) : 96 Wells (OKEH00985)

Catalog No: OKEH00985

Size:96T

Human MSRA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MSRA ELISA Kit (Human) (OKEH00985) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MSRA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MSRA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MSRA captured in well.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 8.0% (n = 20)
• ELISA Kit Component:
  • MSRA Microplate: 96 Wells (12 x 8 Well strips)
  • MSRA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated MSRA Detector Antibody: 1 x 120 ul
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 3.9 pg/mL
• Specificity: Natural and recombinant Human Mitochondrial peptide methionine sulfoxide reductase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MSRA
• Gene Full Name: methionine sulfoxide reductase A
• Alias Symbols: cytosolic methionine-S-sulfoxide reductase;mitochondrial peptide methionine sulfoxide reductase;peptide met (O) reductase;peptide Met(O) reductase;peptide-methionine (S)-S-oxide reductase;PMSR;Protein-methionine-S-oxide reductase.
• NCBI Gene Id: 4482
• Protein Name: Q9UJ68
• Description of Target: Mitochondrial peptide methionine sulfoxide reductase
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UJ68
• Protein Accession #: NP_001129142.1
• Nucleotide Accession #: NM_001135670.2
• Protein Size (# AA): 235
• Molecular Weight: 26 kDa