MSN ELISA Kit (Human) (OKDD00404)

Catalog No: OKDD00404 (Formerly GWB-KBBBU9)

Size:96 Wells

Human MSN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology MSN ELISA Kit (Human) (OKDD00404) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MSN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MSN is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MSN captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MSN were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MSN were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-MSN Microplate: 96 Wells (12 x 8 Well Strips)
  • MSN Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X MSN Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates
• Sensitivity: < 0.064 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of MSN. No significant cross-reactivity or interference between MSN and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MSN
• Gene Full Name: moesin
• Alias Symbols: epididymis luminal protein 70;HEL70;IMD50;membrane-organizing extension spike protein;moesin.
• NCBI Gene Id: 4478
• Protein Name: Moesin
• Description of Target: Probably involved in connections of major cytoskeletal structures to the plasma membrane. May inhibit herpes simplex virus 1 infection at an early stage. Plays a role in regulating the proliferation, migration, and adhesion of human lymphoid cells and participates in immunologic synapse formation (PubMed:27405666).
• Uniprot ID: P26038
• Protein Accession #: NP_002435.1
• Nucleotide Accession #: NM_002444.2
• Protein Size (# AA): 577
• Molecular Weight: 68 kDa