MSH2 Antibody (OAAF03051)

Catalog No: OAAF03051 (Formerly GWB-ASE200)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MSH2 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using MSH2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MSH2.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: MSH2 antibody detects endogenous levels of total MSH2 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000
• Reference: Fishel R., Cell 75:1027-1038(1993).
  Wang Y., Genes Dev. 14:927-939(2000).
  Wang Y., Proc. Natl. Acad. Sci. U.S.A. 100:15387-15392(2003).
• Publications:

  A rapidly growing human papillomavirus-positive oral tongue squamous cell carcinoma in a 21-year old female: A case report. Oncol Lett. 15, 7702-7706 (2018). 29849799

• Description:
  View product citations for MSH2 Antibody (OAAF03051) on CiteAb

Target Info

• Gene Symbol: MSH2
• Gene Full Name: mutS homolog 2
• Alias Symbols: COCA1;DNA mismatch repair protein Msh2;DNA mismatch repair protein Msh2 transcript;FCC1;hMSH2;HNPCC;HNPCC1;LCFS2;MMRCS2;mutS homolog 2, colon cancer, nonpolyposis type 1;MutS protein homolog 2.
• NCBI Gene Id: 4436
• Protein Name: DNA mismatch repair protein Msh2
• Description of Target: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
• Uniprot ID: P43246
• Protein Accession #: NP_000242.1
• Molecular Weight: 104 kDa