Catalog No: OKEH04733
Size:96T
Price: $525.00
SKU
OKEH04733
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ms4a2 ELISA Kit (Rat) (OKEH04733) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ms4a2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ms4a2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ms4a2 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Ms4a2 Microplate96 Wells (12 x 8 Well Strips)
Ms4a2 Standard2
Sample Diluent1 x 20 mL
Ms4a2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity41.5 pg/mL
SpecificityNatural and recombinant Rat High affinity immunoglobulin epsilon receptor subunit beta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMs4a2
Gene Full Namemembrane spanning 4-domains A2
Alias Symbolsbeta polypeptide);fc epsilon receptor I beta-chain;Fcer1b;fcERI;FCIGA;high affinity immunoglobulin epsilon receptor subunit beta;IgE Fc receptor subunit beta;Membrane-spanning 4-domains subfamily A member 2;Membrane-spanning 4-domains, subfamily A, member 1;membrane-spanning 4-domains, subfamily A, member 2 (Fc fragment of IgE, high affinity I, receptor for;membrane-spanning 4-domains, subfamily A, member 2 (Fc fragment of IgE, high affinity I, receptor for; beta polypeptide);Ms4a1.
NCBI Gene Id25316
Protein NameP13386
Description of TargetHigh affinity immunoglobulin epsilon receptor subunit beta
Uniprot IDhttps://www.uniprot.org/uniprot/P13386
Protein Accession #NP_036977.1
Nucleotide Accession #NM_012845.1
Protein Size (# AA)243
Molecular Weight27 kDa
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