MRE11 Antibody (OAPB01017)

Catalog No: OAPB01017

Size:100UG

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Western blot analysis of MRE11 in rat lung tissue lysate with MRE11 antibody at (A) 1 and (B) 2 μg/mL.

Immunohistochemistry of MRE11 in rat lung tissue with MRE11 antibody at 5 μg/mL.

Immunofluorescence of MRE11 in rat lung tissue with MRE11 antibody at 20 μg/mL.

Product Info

• Predicted Species Reactivity: Chicken|Human|Mouse|Rat
• Product Format: Liquid. MRE11 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: MRE11 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: MRE11 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus human MRE11. The immunogen is located within amino acids 40 - 90 of MRE11.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-MRE11 antibody is Catalog # OEPB03133
• Application Info: MRE11 antibody can be used for detection of MRE11 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Stewart GS, Maser RS, Stankovic T, et al. The DNA double-strand break repair gene hMRE11 is mutated in individuals with an ataxia telangiectasia-like disorder. Cell1999; 99:577-87.;Buis J, Wu Y, Deng Y, et al. Mre11 nuclease activity has essential roles in DNA repair and genomic stability distinct from ATM activation. Cell2008; 135:85-96.;

Target Info

• Gene Symbol: MRE11
• Gene Full Name: MRE11 homolog, double strand break repair nuclease
• Alias Symbols: ATLD;AT-like disease;DNA recombination and repair protein;double-strand break repair protein MRE11;double-strand break repair protein MRE11A;endo/exonuclease Mre11;HNGS1;meiotic recombination 11 homolog 1;meiotic recombination 11 homolog A;MRE11 double strand break repair nuclease A;MRE11 homolog 1;MRE11 homolog A, double strand break repair nuclease;MRE11 homolog, double strand break repair nuclease A;MRE11 meiotic recombination 11 homolog A;MRE11 meiotic recombination 11-like protein A;MRE11A;MRE11B.
• NCBI Gene Id: 4361
• Protein Name: Double-strand break repair protein MRE11|MRE11 meiotic recombination 11 homolog A (S. cerevisiae), isoform CRA_a [Homo sapiens]
• Description of Target: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
• Uniprot ID: P49959
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/EAW66932
• Molecular Weight: Predicted: 78 kDa Observed: 73 kDa