MRC1 ELISA Kit (Mouse) (OKCD02731)

Catalog No: OKCD02731

Size:96WELLS

Mouse MRC1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-92%	95-105%	79-92%	79-89%
  EDTA Plasma (n=5)	93-101%	92-101%	80-95%	94-105%
  Heparin Plasma (n=5)	98-105%	83-95%	79-101%	84-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Receptor C Type 1 (MRC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Receptor C Type 1 (MRC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Receptor C Type 1 (MRC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mannose Receptor C Type 1 (MRC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-101	97
  EDTA Plasma (n=5)	79-95	87
  Heparin Plasma(n=5)	96-104	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Receptor C Type 1 (MRC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Receptor C Type 1 (MRC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MRC1 Microplate: 96 Wells (12 x 8 Well strips)
  • MRC1 Lyophilized Standard: 2
  • 100X Biotinylated MRC1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 6.6 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mannose Receptor C Type 1 (MRC1). No significant cross-reactivity or interference between Mannose Receptor C Type 1 (MRC1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mrc1
• Gene Full Name: mannose receptor, C type 1
• Alias Symbols: AW259686;CD206;M;macrophage mannose receptor 1;MMR;MR.
• NCBI Gene Id: 17533
• Protein Name: Macrophage mannose receptor 1
• Description of Target: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
• Uniprot ID: Q61830
• Protein Accession #: NP_032651.2
• Nucleotide Accession #: NM_008625.2
• Protein Size (# AA): 1456